EPatlas is a resource-based database of enhancer RNA and promoter RNA interactions. Enhancers, as cis - regulatory elements, play a pivotal role in the intricate landscape of gene expression regulation. These non - coding DNA sequences, often located distantly from the target genes they regulate, are characterized by their ability to enhance transcriptional activity.

  Based on the RNA in-situ conformation sequencing (RIC-seq) technology, we captured protein-mediated RNA-RNA proximal interactions globally in living cells at a single-nucleotide resolution, which described the interactions between RNAs more precisely. By comparing the identified chimeric reads with the enhancer and promoter intervals defined by Chip-seq data, we can obtain specific information about the interaction between enhancer RNA and promoter RNA.

  The EPatlas currently identified 10 human cell lines and obtained 208,093 high - confidence enhancer RNA and promoter RNA interactions. Among these, the vast majority of interactions are related to the regulation of messenger RNA (mRNA).